- Catalog number
- EK1990
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Alpha- (1,6) -fucosyltransferase is an enzyme that in humans is encoded by the FUT8 gene. It is mapped to 14q23.3. This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 281 268 268 273 5.84 2.1% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 3 4415 4940 4601 4553 4627 193.04 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 281 268 268 273 5.84 2.1% Sample 1 278 281 268 268 273 5.84 2.1% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 2 1061 1124 1190 1141 1129 46.13 4.0% Sample 3 4415 4940 4601 4553 4627 193.04 4.1% Sample 3 4415 4940 4601 4553 4627 193.04 4.1%
- Synonyms
- Alpha- (1,6) -fucosyltransferase; Alpha1-6FucT; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; FUT8
- Gene Name
- FUT8
- Gene ID
- 2530
- UniProt
- Q9BYC5
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R31-K575
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Amino Acid Metabolism, Amino Acids, Cancer, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tumor Biomarkers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin or EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1061 4415 296 1147 4781 Mean (pg/ml) 278 1061 4415 296 1147 4781 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 Standard deviation 13.34 67.90 198.68 20.13 83.73 320.33 CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7% CV (%) 4.8% 6.4% 4.5% 6.8% 7.3% 6.7%
- Sensitivity
- <50 pg/mL
- Function
- Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human FUT8
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fucosyltransferase 8
- Subcellular Location
- Golgi stack membrane. Single-pass type II membrane protein.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058 O.D. 0.051 0.157 0.224 0.365 0.6 1.128 1.551 2.058
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
