- Catalog number
- EK1734
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 9; 9 F1. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 70 64 60 63 4.32 6.8% Sample 2 231 224 225 261 235 15.10 6.4% Sample 3 896 856 928 913 898 26.89 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 70 64 60 63 4.32 6.8% Sample 1 59 70 64 60 63 4.32 6.8% Sample 2 231 224 225 261 235 15.10 6.4% Sample 2 231 224 225 261 235 15.10 6.4% Sample 3 896 856 928 913 898 26.89 2.9% Sample 3 896 856 928 913 898 26.89 2.9%
- Synonyms
- Semaphorin-3F; Semaphorin IV; Sema IV; Sema3f
- Gene Name
- Sema3f
- Gene ID
- 20350
- UniProt
- O88632
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A19-T785
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated detection polyclonal antibody from goat
- Tissue Specificity
- Expressed ubiquitously in adulthood. During embryogenesis, expressed in subregions of the central nervous system and various other tissues like skin, kidney, lung and intestine.
- Applications
- ELISA
- Field of Research
- Axonal Guidance Proteins, Growth and Development, Neurogenesis, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA or citrate)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 231 896 61 245 907 Mean (pg/ml) 59 231 896 61 245 907 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 Standard deviation 3.48 10.16 56.45 4.03 14.21 68.03 CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5% CV (%) 5.9% 4.4% 6.3% 6.6% 5.8% 7.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse SEMA3F
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
- Subcellular Location
- Secreted.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201 O.D. 0.054 0.12 0.186 0.286 0.476 0.828 1.398 2.201
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
