- Catalog number
- EK1930
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 40 42 45 42 1.78 4.2% Sample 2 182 167 169 161 169 7.66 4.5% Sample 3 837 797 842 900 844 36.73 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 40 42 45 42 1.78 4.2% Sample 1 42 40 42 45 42 1.78 4.2% Sample 2 182 167 169 161 169 7.66 4.5% Sample 2 182 167 169 161 169 7.66 4.5% Sample 3 837 797 842 900 844 36.73 4.3% Sample 3 837 797 842 900 844 36.73 4.3%
- Synonyms
- Fc receptor-like protein 1; FcR-like protein 1; FcRL1; Fc receptor homolog 1; FcRH1; IFGP family protein 1; Hifgp1; Immune receptor translocation-associated protein 5; CD307a; FCRL1; FCRH1; IFGP1; IRTA5
- Gene Name
- FCRL1
- Gene ID
- 115350
- UniProt
- Q96LA6
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A17-S307
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Primarily expressed in secondary lymphoid tissues by mature subsets of B-cells. Detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. Specifically expressed by mature B lineage cells with higher expression in naive versus memory B-cells.
- Applications
- ELISA
- Field of Research
- Immunoglobulins, Immunology, Receptors
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 182 837 51 193 947 Mean (pg/ml) 42 182 837 51 193 947 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 Standard deviation 2.18 9.65 46.04 3.11 12.35 63.45 CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7% CV (%) 5.2% 5.3% 5.5% 6.1% 6.4% 6.7%
- Sensitivity
- <20 pg/mL
- Function
- May function as an activating coreceptor in B-cells. May function in B-cells activation and differentiation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human FCRL1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fc receptor like 1
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392 O.D. 0.076 0.15 0.205 0.312 0.52 0.839 1.496 2.392
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
