- Catalog number
- EK2022
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 143 138 129 133 7.11 5.3% Sample 2 689 800 745 802 759 46.43 6.1% Sample 3 2209 2281 2219 2542 2312 135.20 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 125 143 138 129 133 7.11 5.3% Sample 1 125 143 138 129 133 7.11 5.3% Sample 2 689 800 745 802 759 46.43 6.1% Sample 2 689 800 745 802 759 46.43 6.1% Sample 3 2209 2281 2219 2542 2312 135.20 5.8% Sample 3 2209 2281 2219 2542 2312 135.20 5.8%
- Synonyms
- Matrilin-3; MATN3
- Gene Name
- MATN3
- Gene ID
- 4148
- UniProt
- O15232
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D29-R486
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 125 689 2209 148 697 2553 Mean (pg/ml) 125 689 2209 148 697 2553 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 Standard deviation 6.13 29.63 90.57 9.77 40.43 194.03 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6%
- Sensitivity
- <50 pg/mL
- Function
- Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Matrilin-3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Matrilin 3
- Subcellular Location
- Secreted.
- Protein Name
- Matrilin-3
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887 O.D. 0.032 0.077 0.159 0.319 0.638 1.05 1.594 1.887
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
