- Catalog number
- EK2021
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Matrilin-2 is a protein that in humans is encoded by the MATN2 gene. This gene is mapped to 15; 15 B3.1. This gene encodes a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains five von Willebrand factor A domains. The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 194 184 185 185 5.71 3.0% Sample 2 657 670 600 591 629 34.45 5.4% Sample 3 2396 2289 2414 2332 2357 50.04 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 178 194 184 185 185 5.71 3.0% Sample 1 178 194 184 185 185 5.71 3.0% Sample 2 657 670 600 591 629 34.45 5.4% Sample 2 657 670 600 591 629 34.45 5.4% Sample 3 2396 2289 2414 2332 2357 50.04 2.1% Sample 3 2396 2289 2414 2332 2357 50.04 2.1%
- Synonyms
- Matrilin-2; Matn2
- Gene Name
- Matn2
- Gene ID
- 17181
- UniProt
- O08746
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R24-R956
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Detected in a variety of organs, including calvaria, uterus, heart and brain, as well as fibroblast and osteoblast cell lines.
- Applications
- ELISA
- Field of Research
- Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 657 2396 181 685 2417 Standard deviation 8.01 37.45 110.22 9.23 40.42 178.86 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 178 657 2396 181 685 2417 Standard deviation 8.01 37.45 110.22 9.23 40.42 178.86 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 178 657 2396 181 685 2417 Mean (pg/ml) 178 657 2396 181 685 2417 Standard deviation 8.01 37.45 110.22 9.23 40.42 178.86 Standard deviation 8.01 37.45 110.22 9.23 40.42 178.86 CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4% CV (%) 4.5% 5.7% 4.6% 5.1% 5.9% 7.4%
- Sensitivity
- <50 pg/mL
- Function
- Involved in matrix assembly.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse Matrilin-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Matrilin 2
- Subcellular Location
- Secreted.
- Protein Name
- Matrilin-2
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.13 0.242 0.507 0.824 1.311 1.776 2.194 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.13 0.242 0.507 0.824 1.311 1.776 2.194 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.13 0.242 0.507 0.824 1.311 1.776 2.194 O.D. 0.039 0.13 0.242 0.507 0.824 1.311 1.776 2.194
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
