- Catalog number
- EK2035
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45?50 kDa glycoprotein member of the angiopoietin?like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up?regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up?regulated under conditions of increased ocular pressure.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 129 118 129 129 7.77 6.0% Sample 1 140 129 118 129 129 7.77 6.0% Sample 2 644 660 702 583 647 42.71 6.6% Sample 2 644 660 702 583 647 42.71 6.6% Sample 3 1907 2152 2078 2215 2088 115.20 5.5% Sample 3 1907 2152 2078 2215 2088 115.20 5.5%
- Synonyms
- Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356
- Gene Name
- ANGPTL7
- Gene ID
- 10218
- UniProt
- O43827
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: Q27-P346
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Highly and specifically expressed in the cornea where is confined to the stromal layer.
- Applications
- ELISA
- Field of Research
- Neuroscience, Secreted, Sensory System, Signaling Pathways, Stem Cells, Visual System
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 644 1907 145 639 2253 Mean (pg/ml) 140 644 1907 145 639 2253 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%
- Sensitivity
- <50 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ANGPTL7
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Angiopoietin like 7
- Subcellular Location
- Secreted.
- Protein Name
- Angiopoietin-related protein 7
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466 O.D. 0.011 0.089 0.141 0.264 0.521 0.952 1.726 2.466
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
