- Catalog number
- EK2038
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 292 319 286 295 13.82 4.6% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 3 5792 6174 5259 6098 5830 359.73 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 292 319 286 295 13.82 4.6% Sample 1 285 292 319 286 295 13.82 4.6% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 2 1139 1247 1212 1220 1204 39.97 3.3% Sample 3 5792 6174 5259 6098 5830 359.73 6.1% Sample 3 5792 6174 5259 6098 5830 359.73 6.1%
- Synonyms
- Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2
- Gene Name
- TCN2
- Gene ID
- 6948
- UniProt
- P20062
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E19-W427
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cardiovascular, Cofactors, Vitamins/Minerals, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Mean (pg/ml) 285 1139 5792 1123 1123 5755 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 Standard deviation 17.38 72.89 457.56 18.45 74.11 552.48 CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6% CV (%) 6.1% 6.4% 7.9% 6.6% 6.6% 9.6%
- Sensitivity
- <50 pg/mL
- Function
- Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human TCN2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Transcobalamin 2
- Subcellular Location
- Secreted.
- Protein Name
- Transcobalamin-2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938 O.D. 0.002 0.046 0.104 0.244 0.517 0.931 1.546 1.938
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
