- Catalog number
- EK2042
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. The human gene TPP1 encodes a member of the sedolisin family of serine proteases. The human gene has 13 exons and locates at the chromosome band 11p15. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 288 285 315 290 15.63 5.3% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 3 3946 4041 3931 4546 4116 251.81 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 288 285 315 290 15.63 5.3% Sample 1 272 288 285 315 290 15.63 5.3% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 2 1160 1088 1127 980 1088 67.76 6.2% Sample 3 3946 4041 3931 4546 4116 251.81 6.1% Sample 3 3946 4041 3931 4546 4116 251.81 6.1%
- Synonyms
- Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I; TPP1; CLN2; GIG1; UNQ267; PRO304
- Gene Name
- TPP1
- Gene ID
- 1200
- UniProt
- O14773
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L196-P563
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
- Applications
- ELISA
- Field of Research
- Neurogenesis, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1160 3946 303 1187 4281 Mean (pg/ml) 272 1160 3946 303 1187 4281 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 Standard deviation 10.88 54.52 205.19 15.45 70.03 303.95 CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1% CV (%) 4.0% 4.7% 5.2% 5.1% 5.9% 7.1%
- Sensitivity
- <50 pg/mL
- Function
- Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human TPP1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tripeptidyl peptidase 1
- Subcellular Location
- Lysosome. Melanosome.
- Protein Name
- Tripeptidyl-peptidase 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35 O.D. 0.004 0.107 0.207 0.371 0.729 1.374 2.134 2.35
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
