- Catalog number
- EK2001
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. This gene is mapped to 1p36.21. This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 229 278 272 238 254 21.09 8.3% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 3 4782 5075 5245 4924 5006 172.32 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 229 278 272 238 254 21.09 8.3% Sample 1 229 278 272 238 254 21.09 8.3% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 2 1188 1214 1275 1228 1226 31.59 2.5% Sample 3 4782 5075 5245 4924 5006 172.32 3.4% Sample 3 4782 5075 5245 4924 5006 172.32 3.4%
- Synonyms
- Chymotrypsin-C; Caldecrin; CTRC; CLCR
- Gene Name
- CTRC
- Gene ID
- 11330
- UniProt
- Q99895
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: V30-L268
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Pancreas.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 229 1188 4782 314 1249 4914 Mean (pg/ml) 229 1188 4782 314 1249 4914 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 Standard deviation 12.14 66.53 277.36 20.72 86.18 358.72 CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3% CV (%) 5.3% 5.6% 5.8% 6.6% 6.9% 7.3%
- Sensitivity
- <10 pg/mL
- Function
- Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CTRC
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Chymotrypsin C
- Subcellular Location
- Extracellular region.
- Protein Name
- Chymotrypsin-C
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832 O.D. 0.031 0.153 0.25 0.389 0.649 1.033 1.857 2.832
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
