- Catalog number
- EK2003
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Lymphotoxin beta receptor (LTBR), also known as tumor necrosis factor receptor superfamily member 3 (TNFRSF3), is a cell surface receptor for lymphotoxin involved in apoptosis and cytokine release. This gene is mapped to 6 F3; 6 59.32 cM. This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 148 150 127 140 9.33 6.6% Sample 2 552 557 552 596 564 18.44 3.2% Sample 3 2451 2253 2433 2219 2339 103.89 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 148 150 127 140 9.33 6.6% Sample 1 136 148 150 127 140 9.33 6.6% Sample 2 552 557 552 596 564 18.44 3.2% Sample 2 552 557 552 596 564 18.44 3.2% Sample 3 2451 2253 2433 2219 2339 103.89 4.4% Sample 3 2451 2253 2433 2219 2339 103.89 4.4%
- Synonyms
- Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Ltbr; Tnfcr; Tnfrsf3
- Gene Name
- Ltbr
- Gene ID
- 17000
- UniProt
- P50284
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q31-L223
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Apoptosis, Associated Proteins, Cell Biology, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Intracellular, NFKB Pathway, Nuclear Signaling Pathways, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 552 2451 141 582 2471 Standard deviation 7.07 29.26 134.81 8.18 36.08 163.09 CV (%) 5.2% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 552 2451 141 582 2471 Standard deviation 7.07 29.26 134.81 8.18 36.08 163.09 CV (%) 5.2% 5.3% 5.5% 5.8% 6.2% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 136 552 2451 141 582 2471 Mean (pg/ml) 136 552 2451 141 582 2471 Standard deviation 7.07 29.26 134.81 8.18 36.08 163.09 Standard deviation 7.07 29.26 134.81 8.18 36.08 163.09 CV (%) 5.2% 5.3% 5.5% 5.8% 6.2% 6.6% CV (%) 5.2% 5.3% 5.5% 5.8% 6.2% 6.6%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse LTBR
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Lymphotoxin B receptor
- Subcellular Location
- Membrane. Single-pass type I membrane protein.
- Protein Name
- Tumor necrosis factor receptor superfamily member 3
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.088 0.162 0.275 0.484 0.873 1.402 1.963 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.088 0.162 0.275 0.484 0.873 1.402 1.963 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.088 0.162 0.275 0.484 0.873 1.402 1.963 O.D. 0.026 0.088 0.162 0.275 0.484 0.873 1.402 1.963
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
