- Catalog number
- EK2009
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor receptor homolog 3 (TNFRH3; also tumor necrosis factor receptor superfamily member 26) is a 21 kDa (predicted, unglycosylated) type I transmembrane protein and member of the TNF receptor superfamily. This gene is mapped to 7 F5; 7 88.26 cM. The ECD contains two potential sites of N-linked glycosylation and a cysteine-rich region (aa 38?126) . There is no human ortholog for TNFRH3. TNFRH3 is expressed in thymus and spleen with detectable levels in the lung.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 25 27 26 1.08 4.1% Sample 2 112 97 101 113 105 6.90 6.5% Sample 3 460 459 485 428 458 20.21 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 25 27 26 1.08 4.1% Sample 1 28 27 25 27 26 1.08 4.1% Sample 2 112 97 101 113 105 6.90 6.5% Sample 2 112 97 101 113 105 6.90 6.5% Sample 3 460 459 485 428 458 20.21 4.4% Sample 3 460 459 485 428 458 20.21 4.4%
- Synonyms
- Tumor necrosis factor receptor superfamily member 26; TNF receptor homolog 3; Tnfrsf26; Tnfrh3
- Gene Name
- Tnfrsf26
- Gene ID
- 244237
- UniProt
- P83626
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S20-L164
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in thymus and spleen. Detectable levels in lung.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 112 460 30 118 473 Standard deviation 1.12 4.82 18.86 1.68 6.84 34.53 CV (%) 4.0% 4.3% 4.1% 5.6% 5.8% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 112 460 30 118 473 Standard deviation 1.12 4.82 18.86 1.68 6.84 34.53 CV (%) 4.0% 4.3% 4.1% 5.6% 5.8% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 112 460 30 118 473 Mean (pg/ml) 28 112 460 30 118 473 Standard deviation 1.12 4.82 18.86 1.68 6.84 34.53 Standard deviation 1.12 4.82 18.86 1.68 6.84 34.53 CV (%) 4.0% 4.3% 4.1% 5.6% 5.8% 7.3% CV (%) 4.0% 4.3% 4.1% 5.6% 5.8% 7.3%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse TNFRSF26
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily, member 26
- Subcellular Location
- Membrane. Single-pass type I membrane protein.
- Protein Name
- Tumor necrosis factor receptor superfamily member 26
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354 O.D. 0.056 0.123 0.164 0.286 0.527 0.928 1.613 2.354
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
