- Catalog number
- EK2006
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 10q24.31. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 23 24 24 24 1.50 6.2% Sample 2 102 98 104 102 101 2.17 2.1% Sample 3 468 537 490 506 500 25.14 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 23 24 24 24 1.50 6.2% Sample 1 27 23 24 24 24 1.50 6.2% Sample 2 102 98 104 102 101 2.17 2.1% Sample 2 102 98 104 102 101 2.17 2.1% Sample 3 468 537 490 506 500 25.14 5.0% Sample 3 468 537 490 506 500 25.14 5.0%
- Synonyms
- Semaphorin-4G; SEMA4G; KIAA1619
- Gene Name
- SEMA4G
- Gene ID
- 57715
- UniProt
- Q9NTN9
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V18-L675
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Axonal Guidance Proteins, Growth and Development, Neurology Process, Neuroscience
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 102 468 29 114 488 Mean (pg/ml) 27 102 468 29 114 488 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 Standard deviation 1.24 5.10 24.34 1.48 6.73 31.23 CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4% CV (%) 4.6% 5.0% 5.2% 5.1% 5.9% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Cell surface receptor for PLXNB2. May play a role in axon guidance.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human SEMA4G
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Semaphorin 4G
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Protein Name
- Semaphorin-4G
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173 O.D. 0.058 0.141 0.195 0.292 0.509 0.899 1.471 2.173
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
