- Catalog number
- EK2045
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 13 B2; 13 31.92 cM. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transfor
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 573 536 539 600 562 26.31 4.6% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 3 8898 10038 9685 9879 9625 437.95 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 573 536 539 600 562 26.31 4.6% Sample 1 573 536 539 600 562 26.31 4.6% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 2 1930 1980 1951 1871 1933 39.95 2.0% Sample 3 8898 10038 9685 9879 9625 437.95 4.5% Sample 3 8898 10038 9685 9879 9625 437.95 4.5%
- Synonyms
- Growth arrest-specific protein 1; GAS-1; Gas1; Gas-1
- Gene Name
- Gas1
- Gene ID
- 14451
- UniProt
- Q01721
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L39-S315
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 20 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 573 1930 8898 591 2046 9517 Mean (pg/ml) 573 1930 8898 591 2046 9517 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 Standard deviation 23.49 110.01 409.31 34.28 141.17 609.09 CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4% CV (%) 4.1% 5.7% 4.6% 5.8% 6.9% 6.4%
- Sensitivity
- <50 pg/mL
- Function
- Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse GAS1
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Growth arrest specific 1
- Subcellular Location
- Cell membrane. GPI-anchor.
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025 O.D. 0.045 0.137 0.182 0.295 0.496 0.897 1.463 2.025
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
