- Catalog number
- EK2048
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- C-type lectin domain family 10 member A also known as CLEC10A is a protein that in humans is encoded by the CLEC10A gene. This gene is mapped to 17p13.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Sample 3 404 404 433 399 410 13.43 3.2%
- Synonyms
- C-type lectin domain family 10 member A; C-type lectin superfamily member 14; Macrophage lectin 2; CD301; CLEC10A; CLECSF13; CLECSF14; HML
- Gene Name
- CLEC10A
- Gene ID
- 10462
- UniProt
- Q8IUN9
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q61-H316
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5%
- Sensitivity
- <10 pg/mL
- Function
- Probable role in regulating adaptive and innate immune responses. Binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine. These sugar moieties are known as Tn-Ag and are expressed in a variety of carcinoma cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CLEC10A
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- C-type lectin domain containing 10A
- Subcellular Location
- Membrane. Single-pass type II membrane protein.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259 O.D. 0.011 0.061 0.119 0.207 0.401 0.742 1.481 2.259
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
