Human CD206/CLEC13D ELISA Kit PicoKine®

About this product

Human CD206/CLEC13D ELISA Kit PicoKine® (96 Tests) . Quantitate Human MRC1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2049

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205) . This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1229 1161 1088 1126 1151 51.90 4.5% Sample 2 4344 4262 4441 4132 4294 113.32 2.6% Sample 3 20116 20273 22779 21158 21081 1057.49 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1229 1161 1088 1126 1151 51.90 4.5% Sample 1 1229 1161 1088 1126 1151 51.90 4.5% Sample 2 4344 4262 4441 4132 4294 113.32 2.6% Sample 2 4344 4262 4441 4132 4294 113.32 2.6% Sample 3 20116 20273 22779 21158 21081 1057.49 5.0% Sample 3 20116 20273 22779 21158 21081 1057.49 5.0%

Synonyms

Macrophage mannose receptor 1; MMR; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Human mannose receptor; Hmr; Macrophage mannose receptor 1-like protein 1; CD206; MRC1; CLEC13D; CLEC13DL; MRC1L1

Gene Name

MRC1

Gene ID

4360

UniProt

P22897

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-K1383

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1229 4344 20116 1310 4852 23149 Standard deviation 51.62 247.61 885.10 79.91 286.27 1620.43 CV (%) 4.2% 5.7% 4.4% 6.1% 5.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1229 4344 20116 1310 4852 23149 Standard deviation 51.62 247.61 885.10 79.91 286.27 1620.43 CV (%) 4.2% 5.7% 4.4% 6.1% 5.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1229 4344 20116 1310 4852 23149 Mean (pg/ml) 1229 4344 20116 1310 4852 23149 Standard deviation 51.62 247.61 885.10 79.91 286.27 1620.43 Standard deviation 51.62 247.61 885.10 79.91 286.27 1620.43 CV (%) 4.2% 5.7% 4.4% 6.1% 5.9% 7.0% CV (%) 4.2% 5.7% 4.4% 6.1% 5.9% 7.0%

Sensitivity

<50 pg/mL

Function

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. (Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens. (Microbial infection) Acts as a receptor for Dengue virus envelope protein E. (Microbial infection) Interacts with Hepatitis B virus envelope protein.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD206/CLEC13D

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Mannose receptor C-type 1

Subcellular Location

Endosome membrane. Single-pass type I membrane protein. Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.105 0.166 0.305 0.567 0.941 1.586 2.167 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.105 0.166 0.305 0.567 0.941 1.586 2.167 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.105 0.166 0.305 0.567 0.941 1.586 2.167 O.D. 0.018 0.105 0.166 0.305 0.567 0.941 1.586 2.167

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.