- Catalog number
- EK2053
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. This gene is mapped to 12p13.31. This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 27 26 25 25 1.11 4.4% Sample 2 63 62 64 64 63 0.82 1.3% Sample 3 287 292 263 285 281 11.12 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 27 26 25 25 1.11 4.4% Sample 1 24 27 26 25 25 1.11 4.4% Sample 2 63 62 64 64 63 0.82 1.3% Sample 2 63 62 64 64 63 0.82 1.3% Sample 3 287 292 263 285 281 11.12 3.9% Sample 3 287 292 263 285 281 11.12 3.9%
- Synonyms
- Early activation antigen CD69; CD69; Cd69
- Gene Name
- Cd69
- Gene ID
- 12515
- UniProt
- P37217
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N62-R199
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils and platelets.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 63 287 27 84 429 Mean (pg/ml) 24 63 287 27 84 429 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 Standard deviation 1.08 3.34 13.78 1.65 5.38 31.32 CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3% CV (%) 4.5% 5.3% 4.8% 6.1% 6.4% 7.3%
- Sensitivity
- <20 pg/mL
- Function
- Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse CD69
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- CD69 antigen
- Subcellular Location
- Membrane. Single-pass type II membrane protein.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84 O.D. 0.008 0.051 0.108 0.203 0.377 0.672 1.188 1.84
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
