- Catalog number
- EK2054
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 19; 19 C3. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphoring (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite ions.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 2.29 4.3% Sample 2 187 174 190 188 185 11.09 6.0% Sample 3 503 513 506 522 511 32.7 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 2.29 4.3% Sample 1 44 51 58 60 53 2.29 4.3% Sample 2 187 174 190 188 185 11.09 6.0% Sample 2 187 174 190 188 185 11.09 6.0% Sample 3 503 513 506 522 511 32.7 6.4% Sample 3 503 513 506 522 511 32.7 6.4%
- Synonyms
- Semaphorin-4G; Sema4g
- Gene Name
- Sema4g
- Gene ID
- 26456
- UniProt
- Q9WUH7
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V18-M673
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Brain, spinal cord, and several sensory organs as well as specific populations of projection neurons.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 187 503 49 175 512 Mean (pg/ml) 44 187 503 49 175 512 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 Standard deviation 2.16 8.04 20.62 3.23 10.15 38.91 CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.9% 4.3% 4.1% 6.6% 5.8% 7.6%
- Sensitivity
- <10 pg/mL
- Function
- Cell surface receptor for PLXNB2. May play a role in axon guidance.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse SEMA4G
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315 O.D. 0.055 0.113 0.17 0.283 0.49 0.877 1.549 2.315
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
