- Catalog number
- EK2055
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Semaphorin-4G is a protein that in humans is encoded by the SEMA4G gene. This gene is mapped to 1q54. Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphoring (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene and the gene for mitochondrial ribosomal protein L43 overlap at map location 10q24.31 and are transcribed in opposite ions.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 3.57 6.7% Sample 2 95 90 88 85 90 4.3 4.8% Sample 3 133 136 145 158 143 10.3 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 51 58 60 53 3.57 6.7% Sample 1 44 51 58 60 53 3.57 6.7% Sample 2 95 90 88 85 90 4.3 4.8% Sample 2 95 90 88 85 90 4.3 4.8% Sample 3 133 136 145 158 143 10.3 7.2% Sample 3 133 136 145 158 143 10.3 7.2%
- Synonyms
- Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (Semaphorin) 4G (Predicted) ; Semaphorin 4G; Sema4g; Sema4g_predicted; rCG_57705
- Gene Name
- Sema4g
- Gene ID
- 361764
- UniProt
- D4A6G2
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V18-M673
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 95 133 34 91 140 Mean (pg/ml) 44 95 133 34 91 140 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 Standard deviation 1.89 4.94 6.12 2.07 5.37 10.36 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat SEMA4G
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Semaphorin 4G
- Subcellular Location
- Extracellular space. Integral component of plasma membrane.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213 O.D. 0.032 0.175 0.294 0.511 0.851 1.32 1.825 2.213
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
