Rat SEMA6A/Semaphorin-6A ELISA Kit PicoKine®

About this product

Rat SEMA6A ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Sema6a in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2057

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Semaphorin-6A is a protein that in humans is encoded by the SEMA6A gene. This gene is mapped to 18q11. Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 83 88 77 84 4.52 5.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 3 1323 1468 1499 1453 1435 67.17 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 83 88 77 84 4.52 5.3% Sample 1 88 83 88 77 84 4.52 5.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 2 461 388 441 444 433 27.35 6.3% Sample 3 1323 1468 1499 1453 1435 67.17 4.6% Sample 3 1323 1468 1499 1453 1435 67.17 4.6%

Synonyms

Semaphorin 6A; Sema6a

Gene Name

Sema6a

Gene ID

361324

UniProt

D3ZAG0

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-T649

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 461 1323 91 471 1482 Mean (pg/ml) 88 461 1323 91 471 1482 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 Standard deviation 3.52 26.28 60.86 4.64 32.50 103.74 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0%

Sensitivity

<50 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat SEMA6A

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Semaphorin 6A

Subcellular Location

Extracellular space. Integral component of plasma membrane.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724 O.D. 0.02 0.054 0.08 0.139 0.25 0.443 0.906 1.724

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.