- Catalog number
- EK2058
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 3; 3 F2.1. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 85 100 85 91 6.49 7.1% Sample 2 439 446 458 491 458 19.95 4.3% Sample 3 1073 1102 1194 1246 1153 69.51 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 85 100 85 91 6.49 7.1% Sample 1 95 85 100 85 91 6.49 7.1% Sample 2 439 446 458 491 458 19.95 4.3% Sample 2 439 446 458 491 458 19.95 4.3% Sample 3 1073 1102 1194 1246 1153 69.51 6.0% Sample 3 1073 1102 1194 1246 1153 69.51 6.0%
- Synonyms
- Semaphorin-6C; Semaphorin-Y; Sema Y
- Gene Name
- Sema6c
- Gene ID
- 20360
- UniProt
- Q9WTM3
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A26-P605
- Capture Antibody
- A monoclonal antibody from rat
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 439 1073 104 465 1124 Mean (pg/ml) 95 439 1073 104 465 1124 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 Standard deviation 4.56 18.88 43.99 6.86 26.97 85.42 CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6% CV (%) 4.8% 4.3% 4.1% 6.6% 5.8% 7.6%
- Sensitivity
- <50 pg/mL
- Function
- May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant mouse SEMA6C
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174 O.D. 0.052 0.164 0.223 0.316 0.552 0.938 1.413 2.174
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
