- Catalog number
- EK2065
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cytosolic phospholipase A2 is an enzyme that in humans is encoded by the PLA2G4A gene. It is mapped to 1q31.1. This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 100 91 92 4.50 4.8% Sample 2 453 445 454 401 438 21.78 4.9% Sample 3 1183 1364 1244 1343 1283 73.62 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 100 91 92 4.50 4.8% Sample 1 88 91 100 91 92 4.50 4.8% Sample 2 453 445 454 401 438 21.78 4.9% Sample 2 453 445 454 401 438 21.78 4.9% Sample 3 1183 1364 1244 1343 1283 73.62 5.7% Sample 3 1183 1364 1244 1343 1283 73.62 5.7%
- Synonyms
- Cytosolic phospholipase A2; Cpla2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase; PLA2G4A; CPLA2; PLA2G4
- Gene Name
- PLA2G4A
- Gene ID
- 5321
- UniProt
- P47712
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M1-A749
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 453 1183 96 476 1854 Standard deviation 4.31 19.48 60.33 6.34 26.18 142.76 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 453 1183 96 476 1854 Standard deviation 4.31 19.48 60.33 6.34 26.18 142.76 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 453 1183 96 476 1854 Mean (pg/ml) 88 453 1183 96 476 1854 Standard deviation 4.31 19.48 60.33 6.34 26.18 142.76 Standard deviation 4.31 19.48 60.33 6.34 26.18 142.76 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%
- Sensitivity
- <50 pg/mL
- Function
- Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PLA2G4A
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Phospholipase A2 group IVA
- Subcellular Location
- Cytoplasm. Cytoplasmic vesicle.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.133 0.233 0.37 0.655 1.102 1.673 2.383 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.133 0.233 0.37 0.655 1.102 1.673 2.383 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.133 0.233 0.37 0.655 1.102 1.673 2.383 O.D. 0.016 0.133 0.233 0.37 0.655 1.102 1.673 2.383
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
