- Catalog number
- EK2078
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Arylsulfatase B is an enzyme associated with mucopolysaccharidosis VI. It is mapped to 5q14.1. Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 142 155 146 133 144 7.9 5.4% Sample 1 142 155 146 133 144 7.9 5.4% Sample 2 555 583 584 618 585 22.32 3.8% Sample 2 555 583 584 618 585 22.32 3.8% Sample 3 1654 1616 1741 1741 1688 54.67 3.2% Sample 3 1654 1616 1741 1741 1688 54.67 3.2%
- Synonyms
- Arylsulfatase B; ASB; N-acetylgalactosamine-4-sulfatase; G4S; ARSB
- Gene Name
- ARSB
- Gene ID
- 411
- UniProt
- P15848
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S37-M533
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 142 555 1654 131 526 1652 Mean (pg/ml) 142 555 1654 131 526 1652 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 Standard deviation 8.94 25.53 77.73 8.25 29.98 90.86 CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5% CV (%) 6.3% 4.6% 4.7% 6.3% 5.7% 5.5%
- Sensitivity
- <10 pg/mL
- Function
- Removes sulfate groups from chondroitin-4-sulfate (C4S) and regulates its degradation. Involved in the regulation of cell adhesion, cell migration and invasion in colonic epithelium. In the central nervous system, is a regulator of neurite outgrowth and neuronal plasticity, acting through the control of sulfate glycosaminoglycans and neurocan levels.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ARSB
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Arylsulfatase B
- Subcellular Location
- Lysosome. Cell surface.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317 O.D. 0.002 0.102 0.189 0.36 0.66 1.042 1.707 2.317
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
