- Catalog number
- EK2080
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 2q36.3. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 76 117 106 94 3.65 3.9% Sample 2 174 203 247 148 193 11.77 6.1% Sample 3 1710 1611 1200 1578 1525 95.6 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 76 117 106 94 3.65 3.9% Sample 1 75 76 117 106 94 3.65 3.9% Sample 2 174 203 247 148 193 11.77 6.1% Sample 2 174 203 247 148 193 11.77 6.1% Sample 3 1710 1611 1200 1578 1525 95.6 6.3% Sample 3 1710 1611 1200 1578 1525 95.6 6.3%
- Synonyms
- Delta and Notch-like epidermal growth factor-related receptor; DNER; BET; UNQ262; PRO299
- Gene Name
- DNER
- Gene ID
- 92737
- UniProt
- Q8NFT8
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N35-Y640
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in brain, spinal cord and adrenal gland.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2%
- Sensitivity
- <30 pg/mL
- Function
- Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human DNER
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Delta/notch like EGF repeat containing
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
