- Catalog number
- EK1765
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Peptidyl-glycine alpha-amidating monooxygenase is an enzyme that catalyzes the biosynthesis of many signaling peptides and, in humans, is encoded by the PAM gene. It is mapped to 5q21.1. This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 228 291 184 217 4.51 2.1% Sample 2 572 532 446 618 542 39.73 7.3% Sample 3 4574 4967 4068 4322 4483 147.48 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 228 291 184 217 4.51 2.1% Sample 1 164 228 291 184 217 4.51 2.1% Sample 2 572 532 446 618 542 39.73 7.3% Sample 2 572 532 446 618 542 39.73 7.3% Sample 3 4574 4967 4068 4322 4483 147.48 3.3% Sample 3 4574 4967 4068 4322 4483 147.48 3.3%
- Synonyms
- Peptidyl-glycine alpha-amidating monooxygenase; PAM; Peptidylglycine alpha-hydroxylating monooxygenase; PHM; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; Peptidylamidoglycolate lyase; PAL; PAM
- Gene Name
- PAM
- Gene ID
- 5066
- UniProt
- P19021
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M1-V817
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 572 4574 250 468 3375 Standard deviation 4.1 31.17 116.18 11.65 34.77 146.48 CV (%) 2.5% 5.5% 2.5% 4.7% 7.4% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 572 4574 250 468 3375 Standard deviation 4.1 31.17 116.18 11.65 34.77 146.48 CV (%) 2.5% 5.5% 2.5% 4.7% 7.4% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 164 572 4574 250 468 3375 Mean (pg/ml) 164 572 4574 250 468 3375 Standard deviation 4.1 31.17 116.18 11.65 34.77 146.48 Standard deviation 4.1 31.17 116.18 11.65 34.77 146.48 CV (%) 2.5% 5.5% 2.5% 4.7% 7.4% 4.3% CV (%) 2.5% 5.5% 2.5% 4.7% 7.4% 4.3%
- Sensitivity
- <75 pg/mL
- Function
- Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl (2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PAM
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Peptidylglycine alpha-amidating monooxygenase
- Subcellular Location
- Membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.119 0.173 0.283 0.494 0.822 1.396 2.23 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.119 0.173 0.283 0.494 0.822 1.396 2.23 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.045 0.119 0.173 0.283 0.494 0.822 1.396 2.23 O.D. 0.045 0.119 0.173 0.283 0.494 0.822 1.396 2.23
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
