Human Contactin-4 ELISA Kit PicoKine®

About this product

Human Contactin-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CNTN4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1780

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 3p26.3-p26.2. This gene encodes a member of the contactin family of immunoglobulins. Contactins are axon-associated cell adhesion molecules that function in neuronal network formation and plasticity. The encoded protein is a glycosylphosphatidylinositol-anchored neuronal membrane protein that may play a role in the formation of axon connections in the developing nervous system. Deletion or mutation of this gene may play a role in 3p deletion syndrome and autism spectrum disorders. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 376 320 370 352 22.38 6.3% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 3 8803 9355 8126 9099 8845 459.16 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 376 320 370 352 22.38 6.3% Sample 1 343 376 320 370 352 22.38 6.3% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 2 1884 1754 1627 1875 1785 104.67 5.8% Sample 3 8803 9355 8126 9099 8845 459.16 5.1% Sample 3 8803 9355 8126 9099 8845 459.16 5.1%

Synonyms

Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; CNTN4

Gene Name

CNTN4

Gene ID

152330

UniProt

Q8IWV2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S1000

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 343 1884 8803 425 2121 9479 Mean (pg/ml) 343 1884 8803 425 2121 9479 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 Standard deviation 14.75 97.97 404.94 25.93 125.14 701.45 CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4% CV (%) 4.3% 5.2% 4.6% 6.1% 5.9% 7.4%

Sensitivity

<50 pg/mL

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Contactin-4

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Contactin 4

Subcellular Location

Secreted. Cell membrane. GPI-anchor.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272 O.D. 0.049 0.126 0.193 0.285 0.488 0.874 1.516 2.272

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.