Mouse Contactin-4 ELISA Kit PicoKine®

About this product

Mouse Contactin-4 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cntn4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1781

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Contactin-4 is a protein that in humans is encoded by the CNTN4 gene. It is mapped to 6; 6 E1. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 451 511 512 479 488 25.27 5.1% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 3 7615 7780 8028 8212 7908 228.60 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 451 511 512 479 488 25.27 5.1% Sample 1 451 511 512 479 488 25.27 5.1% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 2 1849 2120 1840 2095 1976 131.83 6.6% Sample 3 7615 7780 8028 8212 7908 228.60 2.8% Sample 3 7615 7780 8028 8212 7908 228.60 2.8%

Synonyms

Contactin-4; Brain-derived immunoglobulin superfamily protein 2; BIG-2; Cntn4; Kiaa3024

Gene Name

Cntn4

Gene ID

269784

UniProt

Q69Z26

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S1000

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in the region of developing olfactory neurons. Isoform 2 is expressed in mature sensory cells of the vomeronasal neuroepithelium and at lower level in olfactory neuroepithelium.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 451 1849 7615 513 2075 8739 Mean (pg/ml) 451 1849 7615 513 2075 8739 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 Standard deviation 26.61 79.51 312.22 33.86 118.28 681.64 CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8% CV (%) 5.9% 4.3% 4.1% 6.6% 5.7% 7.8%

Sensitivity

<50 pg/mL

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Contactin-4

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Contactin 4

Subcellular Location

Secreted. Cell membrane. GPI-anchor.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95 O.D. 0.053 0.138 0.192 0.321 0.509 0.902 1.476 1.95

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.