Human Contactin-5 ELISA Kit PicoKine®

About this product

Human Contactin-5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CNTN5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1782

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Contactin-5 is a protein that in humans is encoded by the CNTN5 gene. It is mapped to 11q22.1. The protein encoded by this gene is a member of the immunoglobulin superfamily, and contactin family, which mediate cell surface interactions during nervous system development. This protein is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 302 291 294 288 294 12.34 4.2% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 3 6356 5997 6413 6982 6437 321.85 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 302 291 294 288 294 12.34 4.2% Sample 1 302 291 294 288 294 12.34 4.2% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 2 1651 1636 1744 1596 1657 125.91 7.6% Sample 3 6356 5997 6413 6982 6437 321.85 5.0% Sample 3 6356 5997 6413 6982 6437 321.85 5.0%

Synonyms

Contactin-5; Neural recognition molecule NB-2; hNB-2; CNTN5

Gene Name

CNTN5

Gene ID

53942

UniProt

O94779

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-S1072

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 302 1651 6356 294 1742 6883 Mean (pg/ml) 302 1651 6356 294 1742 6883 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 Standard deviation 12.08 94.11 292.38 14.99 122.2 481.81 CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0% CV (%) 4.0% 5.7% 4.6% 5.1% 6.9% 7.0%

Sensitivity

<50 pg/mL

Function

Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity in the cerebral cortical neurons but not in hippocampal neurons. Probably involved in neuronal activity in the auditory system.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Contactin-5

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Contactin 5

Subcellular Location

Expressed in brain and kidney and at very low level in placenta. Not expressed in other tissues. In brain, it is highly expressed in the occipital lobe, amygdala, cerebral cortex, frontal lobe, thalamus and temporal lobe. Expressed at moderate level in the cerebellum, substantia nigra, putamen, medulla and hippocampus. Weakly expressed in the spinal cord and caudate nucleus. Weakly or not expressed in the corpus callosum.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646 O.D. 0.046 0.096 0.138 0.244 0.422 0.843 1.607 2.646

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.