Human SPOCK1/Testican-1 ELISA Kit PicoKine®

About this product

Human SPOCK1/Testican-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SPOCK1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1848

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Testican-1 is a protein that in humans is encoded by the SPOCK1 gene. It is mapped to 5q31.2. This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 23 22 20 21 1.11 5.2% Sample 2 67 71 74 66 69 3.20 4.6% Sample 3 435 409 375 396 403 21.74 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 23 22 20 21 1.11 5.2% Sample 1 21 23 22 20 21 1.11 5.2% Sample 2 67 71 74 66 69 3.20 4.6% Sample 2 67 71 74 66 69 3.20 4.6% Sample 3 435 409 375 396 403 21.74 5.3% Sample 3 435 409 375 396 403 21.74 5.3%

Synonyms

Testican-1; Protein SPOCK; SPOCK1; SPOCK; TIC1; TICN1

Gene Name

SPOCK1

Gene ID

6695

UniProt

Q08629

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R22-W439

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 67 435 26 73 474 Standard deviation 0.95 2.88 22.19 1.72 5.04 34.60 CV (%) 4.5% 4.3% 5.1% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 67 435 26 73 474 Standard deviation 0.95 2.88 22.19 1.72 5.04 34.60 CV (%) 4.5% 4.3% 5.1% 6.6% 6.9% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 21 67 435 26 73 474 Mean (pg/ml) 21 67 435 26 73 474 Standard deviation 0.95 2.88 22.19 1.72 5.04 34.60 Standard deviation 0.95 2.88 22.19 1.72 5.04 34.60 CV (%) 4.5% 4.3% 5.1% 6.6% 6.9% 7.3% CV (%) 4.5% 4.3% 5.1% 6.6% 6.9% 7.3%

Sensitivity

<10 pg/mL

Function

May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human SPOCK1/Testican-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1

Subcellular Location

Extracellular matrix.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.035 0.066 0.111 0.199 0.38 0.671 1.105 1.803 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.035 0.066 0.111 0.199 0.38 0.671 1.105 1.803 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.035 0.066 0.111 0.199 0.38 0.671 1.105 1.803 O.D. 0.035 0.066 0.111 0.199 0.38 0.671 1.105 1.803

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.