- Catalog number
- EK1850
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cell surface transmembrane glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene. It is mapped to 3q13.2. This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 26 23 23 24 1.29 5.3% Sample 2 97 110 101 94 100 6.02 6.0% Sample 3 433 436 409 424 425 10.50 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 26 23 23 24 1.29 5.3% Sample 1 25 26 23 23 24 1.29 5.3% Sample 2 97 110 101 94 100 6.02 6.0% Sample 2 97 110 101 94 100 6.02 6.0% Sample 3 433 436 409 424 425 10.50 2.4% Sample 3 433 436 409 424 425 10.50 2.4%
- Synonyms
- Cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Cell surface glycoprotein OX2 receptor 1; CD200R1; CD200R; CRTR2; MOX2R; OX2R; UNQ2522; PRO6015
- Gene Name
- CD200R1
- Gene ID
- 131450
- UniProt
- Q8TD46
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M29-L243
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Expressed in granulocytes, monocytes, most T-cells, neutrophils, basophils and a subset of NK, NKT and B-cells (at protein level) . Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 97 433 26 103 468 Standard deviation 1.30 4.17 17.75 1.72 5.97 30.89 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 97 433 26 103 468 Standard deviation 1.30 4.17 17.75 1.72 5.97 30.89 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 25 97 433 26 103 468 Mean (pg/ml) 25 97 433 26 103 468 Standard deviation 1.30 4.17 17.75 1.72 5.97 30.89 Standard deviation 1.30 4.17 17.75 1.72 5.97 30.89 CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6% CV (%) 5.2% 4.3% 4.1% 6.6% 5.8% 6.6%
- Sensitivity
- <10 pg/mL
- Function
- Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CD200R1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD200 receptor 1
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein. Secreted.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.073 0.116 0.159 0.254 0.397 0.687 1.251 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.073 0.116 0.159 0.254 0.397 0.687 1.251 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.073 0.116 0.159 0.254 0.397 0.687 1.251 2.085 O.D. 0.073 0.116 0.159 0.254 0.397 0.687 1.251 2.085
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
