- Catalog number
- EK1851
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Ectodysplasin A receptor (EDAR) is a protein that in humans is encoded by the EDAR gene. It is mapped to 2q13. This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 20 21 22 21 0.70 3.3% Sample 2 75 91 75 75 79 6.92 8.7% Sample 3 384 433 444 406 416 23.42 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 21 20 21 22 21 0.70 3.3% Sample 1 21 20 21 22 21 0.70 3.3% Sample 2 75 91 75 75 79 6.92 8.7% Sample 2 75 91 75 75 79 6.92 8.7% Sample 3 384 433 444 406 416 23.42 5.6% Sample 3 384 433 444 406 416 23.42 5.6%
- Synonyms
- Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless homolog; EDA-A1 receptor; Ectodermal dysplasia receptor; Ectodysplasin-A receptor; EDAR; DL
- Gene Name
- EDAR
- Gene ID
- 10913
- UniProt
- Q9UNE0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: E27-A187
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Detected in fetal kidney, lung, skin and cultured neonatal epidermal keratinocytes. Not detected in lymphoblast and fibroblast cell lines.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 21 75 384 29 120 428 Mean (pg/ml) 21 75 384 29 120 428 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 Standard deviation 0.88 3.30 17.66 1.48 7.08 27.39 CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4% CV (%) 4.2% 4.4% 4.6% 5.1% 5.9% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human EDAR
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Ectodysplasin A receptor
- Subcellular Location
- Membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
