- Catalog number
- EK1852
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Von Willebrand factor, type C (VWFC or VWC) is a protein domain is found in various blood plasma proteins: complement factors B, C2, CR3 and CR4; the integrins (I-domains) ; collagen types VI, VII, XII and XIV; and other extracellular proteins. It is mapped to 7p12.2. This gene encodes a secreted bone morphogenic protein antagonist. The encoded protein is possibly involved in neural function and development and may have a role in cell adhesion.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 23 28 26 25 1.92 7.6% Sample 2 73 76 83 77 77 3.63 4.7% Sample 3 427 447 399 461 433 23.29 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 23 28 26 25 1.92 7.6% Sample 1 24 23 28 26 25 1.92 7.6% Sample 2 73 76 83 77 77 3.63 4.7% Sample 2 73 76 83 77 77 3.63 4.7% Sample 3 427 447 399 461 433 23.29 5.3% Sample 3 427 447 399 461 433 23.29 5.3%
- Synonyms
- Brorin; Brain-specific chordin-like protein; von Willebrand factor C domain-containing protein 2; VWC2; UNQ739; PRO1434
- Gene Name
- VWC2
- Gene ID
- 375567
- UniProt
- Q2TAL6
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S28-M325
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 73 427 26 84 437 Standard deviation 1.18 3.14 21.78 1.72 4.62 33.65 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 73 427 26 84 437 Standard deviation 1.18 3.14 21.78 1.72 4.62 33.65 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 73 427 26 84 437 Mean (pg/ml) 24 73 427 26 84 437 Standard deviation 1.18 3.14 21.78 1.72 4.62 33.65 Standard deviation 1.18 3.14 21.78 1.72 4.62 33.65 CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7% CV (%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%
- Sensitivity
- <10 pg/mL
- Function
- BMP antagonist which may play a role in neural development. Promotes cell adhesion.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Brorin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Von Willebrand factor C domain containing 2
- Subcellular Location
- Basement membrane. Synapse.
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.132 0.232 0.397 0.709 1.235 1.748 2.076 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.132 0.232 0.397 0.709 1.235 1.748 2.076 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.132 0.232 0.397 0.709 1.235 1.748 2.076 O.D. 0.051 0.132 0.232 0.397 0.709 1.235 1.748 2.076
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
