- Catalog number
- EK2096
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 5p13.3. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 180 203 210 195 11.86 6.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 3 3740 3185 3099 3077 3275 271.34 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 180 203 210 195 11.86 6.0% Sample 1 188 180 203 210 195 11.86 6.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 2 813 747 779 790 782 23.76 3.0% Sample 3 3740 3185 3099 3077 3275 271.34 8.2% Sample 3 3740 3185 3099 3077 3275 271.34 8.2%
- Synonyms
- Cadherin-6; Kidney cadherin; K-cadherin; CDH6
- Gene Name
- CDH6
- Gene ID
- 1004
- UniProt
- P55285
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S54-A615
- Capture Antibody
- A polyclonal antibody from sheep
- Detection Antibody
- A biotinylated polyclonal antibody from sheep
- Tissue Specificity
- Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 813 3740 214 954 4861 Mean (pg/ml) 188 813 3740 214 954 4861 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 Standard deviation 7.71 34.96 172.04 10.91 56.29 325.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7%
- Sensitivity
- <50 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human Cadherin-6
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Cadherin 6
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008 O.D. 0.025 0.072 0.117 0.2 0.414 0.688 1.221 2.008
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
