- Catalog number
- EK2098
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cadherin-6 is a protein that in humans is encoded by the CDH6 gene. It is mapped to 2q16. This gene encodes a member of the cadherin superfamily. Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation. Decreased expression of this gene may be associated with tumor growth and metastasis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 215 198 199 201 7.79 3.8% Sample 2 980 991 887 890 937 48.66 5.1% Sample 3 3616 3729 3935 3632 3728 127.09 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 215 198 199 201 7.79 3.8% Sample 1 195 215 198 199 201 7.79 3.8% Sample 2 980 991 887 890 937 48.66 5.1% Sample 2 980 991 887 890 937 48.66 5.1% Sample 3 3616 3729 3935 3632 3728 127.09 3.4% Sample 3 3616 3729 3935 3632 3728 127.09 3.4%
- Synonyms
- Cadherin-6; Kidney cadherin; K-cadherin; Cdh6; Kcad
- Gene Name
- Cdh6
- Gene ID
- 25409
- UniProt
- P55280
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S54-A615
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in kidney and brain.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 195 980 3616 268 1119 4324 Mean (pg/ml) 195 980 3616 268 1119 4324 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 Standard deviation 8.19 42.14 148.26 17.69 80.57 328.62 CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6% CV (%) 4.2% 4.3% 4.1% 6.6% 7.2% 7.6%
- Sensitivity
- <50 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat Cadherin-6
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Cadherin 6
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256 O.D. 0.057 0.101 0.156 0.256 0.456 0.896 1.592 2.256
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
