- Catalog number
- EK2101
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fms-related tyrosine kinase 3 ligand (FLT3LG) is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 1q22. Flt3 ligand (FL) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1) . In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. For example, it is a major growth factor stimulating the growth of dendritic cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 33 34 35 1.58 4.5% Sample 2 160 162 184 182 172 11.04 6.4% Sample 3 699 680 621 637 659 31.49 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 33 34 35 1.58 4.5% Sample 1 36 37 33 34 35 1.58 4.5% Sample 2 160 162 184 182 172 11.04 6.4% Sample 2 160 162 184 182 172 11.04 6.4% Sample 3 699 680 621 637 659 31.49 4.7% Sample 3 699 680 621 637 659 31.49 4.7%
- Synonyms
- Fms-related tyrosine kinase 3 ligand; Flt3lg
- Gene Name
- Flt3lg
- Gene ID
- 103691134
- UniProt
- A0A1W2Q6J3
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G27-R188
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 160 699 41 184 759 Mean (pg/ml) 36 160 699 41 184 759 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 Standard deviation 1.62 6.88 28.66 2.46 10.67 49.34 CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5% CV (%) 4.5% 4.3% 4.1% 6.0% 5.8% 6.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat Flt-3 ligand
- Gene Name Synonym
- Fms-related tyrosine kinase 3 ligand
- Subcellular Location
- Extracellular space. Intrinsic component of external side of plasma membrane. Integral component of membrane.
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565 O.D. 0.015 0.076 0.134 0.239 0.419 0.824 1.457 2.565
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
