- Catalog number
- EK0545
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 268 282 273 286 277 7.11 2.5% Sample 2 670 653 609 610 635 26.68 4.2% Sample 3 2924 3021 2641 3116 2925 177.73 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 268 282 273 286 277 7.11 2.5% Sample 1 268 282 273 286 277 7.11 2.5% Sample 2 670 653 609 610 635 26.68 4.2% Sample 2 670 653 609 610 635 26.68 4.2% Sample 3 2924 3021 2641 3116 2925 177.73 6.0% Sample 3 2924 3021 2641 3116 2925 177.73 6.0%
- Synonyms
- Vascular endothelial growth factor receptor 3; VEGFR-3; Fms-like tyrosine kinase 4; FLT-4; Tyrosine-protein kinase receptor FLT4; FLT4; VEGFR3
- Gene Name
- FLT4
- Gene ID
- 2324
- UniProt
- P35916
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Y25-I776
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Tissue Specificity
- Detected in endothelial cells (at protein level) . Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 670 2924 294 854 3861 Standard deviation 10.99 28.81 134.50 14.99 50.39 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 670 2924 294 854 3861 Standard deviation 10.99 28.81 134.50 14.99 50.39 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 268 670 2924 294 854 3861 Mean (pg/ml) 268 670 2924 294 854 3861 Standard deviation 10.99 28.81 134.50 14.99 50.39 258.69 Standard deviation 10.99 28.81 134.50 14.99 50.39 258.69 CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7% CV (%) 4.1% 4.3% 4.6% 5.1% 5.9% 6.7%
- Sensitivity
- <50 pg/mL
- Function
- Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human FLT4/VEGFR3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fms related tyrosine kinase 4
- Subcellular Location
- Cell membrane. Single-pass type I membrane protein. Nucleus. Cytoplasm.
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.098 0.153 0.289 0.498 0.877 1.435 1.967 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.098 0.153 0.289 0.498 0.877 1.435 1.967 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.098 0.153 0.289 0.498 0.877 1.435 1.967 O.D. 0.044 0.098 0.153 0.289 0.498 0.877 1.435 1.967
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
