- Catalog number
- EK2109
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CANT1?is a?gene?that encodes soluble calcium-activated?nucleotidase?1, an?enzyme, in humans. It is mapped to 17q25.3. This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 319 341 313 295 317 16.43 5.1% Sample 1 319 341 313 295 317 16.43 5.1% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 2 1085 1189 1030 1018 1080 67.54 6.2% Sample 3 5570 5244 5973 5762 5637 268.07 4.7% Sample 3 5570 5244 5973 5762 5637 268.07 4.7%
- Synonyms
- Soluble calcium-activated nucleotidase 1; SCAN-1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; CANT1; SHAPY
- Gene Name
- CANT1
- Gene ID
- 124583
- UniProt
- Q8WVQ1
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: R63-I401
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated monoclonal antibody from goat
- Tissue Specificity
- Widely expressed.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 301 1170 4798 307 1195 4812 Mean (pg/ml) 301 1170 4798 307 1195 4812 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 Standard deviation 15.65 62.01 259.09 18.73 70.51 327.22 CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8% CV (%) 5.2% 5.3% 5.4% 6.1% 5.9% 6.8%
- Sensitivity
- <50 pg/mL
- Function
- Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15248776, PubMed:15006348, PubMed:16835225) . Involved in proteoglycan synthesis (PubMed:22539336) .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human CANT1
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Calcium activated nucleotidase 1
- Subcellular Location
- Endoplasmic reticulum membrane; Single-pass type II membrane protein; Golgi stack membrane; Single-pass type II membrane protein; Cell membrane
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265 O.D. 0.015 0.076 0.134 0.239 0.509 0.904 1.457 2.265
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
