- Catalog number
- EK2110
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Prolargin?is a?protein?that in humans is encoded by the?PRELP?gene. It is mapped to 1q32.1. The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 112 116 125 118 5.06 4.2% Sample 1 122 112 116 125 118 5.06 4.2% Sample 2 725 748 679 765 729 32.29 4.4% Sample 2 725 748 679 765 729 32.29 4.4% Sample 3 2351 2384 2122 2446 2325 122.47 5.2% Sample 3 2351 2384 2122 2446 2325 122.47 5.2%
- Synonyms
- Prolargin; Proline-arginine-rich end leucine-rich repeat protein; PRELP; SLRR2A
- Gene Name
- PRELP
- Gene ID
- 5549
- UniProt
- P51888
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q21-I382
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated monoclonal antibody from goat
- Tissue Specificity
- Connective tissue.
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 93.8 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 740 3579 195 936 3857 Standard deviation 5.30 48.10 204.00 13.46 54.29 242.99 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 740 3579 195 936 3857 Standard deviation 5.30 48.10 204.00 13.46 54.29 242.99 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 740 3579 195 936 3857 Mean (pg/ml) 102 740 3579 195 936 3857 Standard deviation 5.30 48.10 204.00 13.46 54.29 242.99 Standard deviation 5.30 48.10 204.00 13.46 54.29 242.99 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3%
- Sensitivity
- <50 pg/mL
- Function
- May anchor basement membranes to the underlying connective tissue.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human PRELP
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Proline and arginine rich end leucine rich repeat protein
- Subcellular Location
- Extracellular matrix
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059 O.D. 0.062 0.144 0.218 0.378 0.612 0.939 1.446 2.059
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
