Mouse ANTXR2 ELISA Kit PicoKine®

About this product

Mouse ANTXR2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2122

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Anthrax toxin receptor 2 is a protein that in humans is encoded by the ANTXR2 gene. It is mapped to 5; 5 E3. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 52 54 43 51 1.54 3.0% Sample 2 178 144 192 214 182 10.70 5.9% Sample 3 853 930 631 618 758 54.88 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 52 54 43 51 1.54 3.0% Sample 1 53 52 54 43 51 1.54 3.0% Sample 2 178 144 192 214 182 10.70 5.9% Sample 2 178 144 192 214 182 10.70 5.9% Sample 3 853 930 631 618 758 54.88 7.2% Sample 3 853 930 631 618 758 54.88 7.2%

Synonyms

Anthrax toxin receptor 2; Antxr2

Gene Name

ANTXR2

Gene ID

71914

UniProt

Q6DFX2

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-G318

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated monoclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL -2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 178 853 61 249 563 Standard deviation 1.47 6.55 22.86 1.32 13.79 16.21 CV (%) 2.8% 3.7% 2.7% 2.2% 5.5% 2.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 178 853 61 249 563 Standard deviation 1.47 6.55 22.86 1.32 13.79 16.21 CV (%) 2.8% 3.7% 2.7% 2.2% 5.5% 2.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 178 853 61 249 563 Mean (pg/ml) 53 178 853 61 249 563 Standard deviation 1.47 6.55 22.86 1.32 13.79 16.21 Standard deviation 1.47 6.55 22.86 1.32 13.79 16.21 CV (%) 2.8% 3.7% 2.7% 2.2% 5.5% 2.9% CV (%) 2.8% 3.7% 2.7% 2.2% 5.5% 2.9%

Sensitivity

<50 pg/mL

Function

Necessary for cellular interactions with laminin and the extracellular matrix.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse ANTXR2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Anthrax toxin receptor 2

Subcellular Location

Membrane. Single-pass type I membrane protein.

Protein Name

Anthrax toxin receptor 2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.094 0.155 0.273 0.498 0.918 1.684 2.751 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.094 0.155 0.273 0.498 0.918 1.684 2.751 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.022 0.094 0.155 0.273 0.498 0.918 1.684 2.751 O.D. 0.022 0.094 0.155 0.273 0.498 0.918 1.684 2.751

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.