Mouse SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®

About this product

Mouse SPARCL1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse SPARCL1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2125

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. It is mapped to 5 E5; 5 50.55 cM. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 467 427 448 472 453 17.72 3.9% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 3 7832 7176 7216 8274 7624 456.32 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 467 427 448 472 453 17.72 3.9% Sample 1 467 427 448 472 453 17.72 3.9% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 2 1310 1396 1545 1357 1402 87.99 6.2% Sample 3 7832 7176 7216 8274 7624 456.32 5.9% Sample 3 7832 7176 7216 8274 7624 456.32 5.9%

Synonyms

SPARC-like protein 1; Extracellular matrix protein 2; Matrix glycoprotein Sc1; Sparcl1; Ecm2; Sc1

Gene Name

Sparcl1

Gene ID

13602

UniProt

P70663

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I17-F650

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Highest expression in brain. Moderate levels in heart, adrenal gland, epididymis and lung. Low levels in kidney, eye, liver, spleen, submandibular gland and testis.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 467 1310 7832 529 2102 8406 Mean (pg/ml) 467 1310 7832 529 2102 8406 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 Standard deviation 19.15 56.33 360.27 30.68 145.04 605.23 CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2% CV (%) 4.1% 4.3% 4.6% 5.8% 6.9% 7.2%

Sensitivity

<50 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SPARCL1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

SPARC-like 1

Subcellular Location

Extracellular matrix.

Protein Name

SPARC-like protein 1

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902 O.D. 0.002 0.058 0.092 0.17 0.319 0.599 1.08 1.902

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.