Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine®

About this product

Mouse Crossveinless-2/CV-2/BMPER ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Bmper in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2119

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. Mutations in this gene are associated with a lethal skeletal disorder, diaphanospondylodysostosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 207 220 215 195 209 7.94 3.8% Sample 2 813 827 854 792 822 46.85 5.7% Sample 3 1459 1322 1478 1334 1398 86.68 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 207 220 215 195 209 7.94 3.8% Sample 1 207 220 215 195 209 7.94 3.8% Sample 2 813 827 854 792 822 46.85 5.7% Sample 2 813 827 854 792 822 46.85 5.7% Sample 3 1459 1322 1478 1334 1398 86.68 6.2% Sample 3 1459 1322 1478 1334 1398 86.68 6.2%

Gene Name

Bmper

Gene ID

73230

UniProt

Q8CJ69

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V34-R685

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 207 813 1459 212 846 1558 Standard deviation 11.18 46.34 97.75 12.30 62.60 123.08 CV (%) 5.4% 5.7% 6.7% 5.8% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 207 813 1459 212 846 1558 Standard deviation 11.18 46.34 97.75 12.30 62.60 123.08 CV (%) 5.4% 5.7% 6.7% 5.8% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 207 813 1459 212 846 1558 Mean (pg/ml) 207 813 1459 212 846 1558 Standard deviation 11.18 46.34 97.75 12.30 62.60 123.08 Standard deviation 11.18 46.34 97.75 12.30 62.60 123.08 CV (%) 5.4% 5.7% 6.7% 5.8% 7.4% 7.9% CV (%) 5.4% 5.7% 6.7% 5.8% 7.4% 7.9%

Sensitivity

<50 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse BMPER

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.097 0.185 0.342 0.648 1.063 1.625 2.211 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.097 0.185 0.342 0.648 1.063 1.625 2.211 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.019 0.097 0.185 0.342 0.648 1.063 1.625 2.211 O.D. 0.019 0.097 0.185 0.342 0.648 1.063 1.625 2.211

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.