Human NCAN/Neurocan ELISA Kit PicoKine®

About this product

Human NCAN/Neurocan ELISA Kit PicoKine® (96 Tests) . Quantitate Human NCAN in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2116

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 88 85 87 4.52 5.2% Sample 2 221 258 211 247 234 18.02 7.7% Sample 3 972 965 933 954 956 80.30 8.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 91 88 85 87 4.52 5.2% Sample 1 83 91 88 85 87 4.52 5.2% Sample 2 221 258 211 247 234 18.02 7.7% Sample 2 221 258 211 247 234 18.02 7.7% Sample 3 972 965 933 954 956 80.30 8.4% Sample 3 972 965 933 954 956 80.30 8.4%

Gene Name

NCAN

Gene ID

1463

UniProt

O14594

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E23-C1321

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 221 972 75 236 860 Mean (pg/ml) 83 221 972 75 236 860 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 Standard deviation 5.06 16.13 58.32 5.10 21.24 66.22 CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7% CV (%) 6.1% 7.3% 6% 6.8% 9% 7.7%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NCAN

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498 O.D. 0.059 0.143 0.203 0.307 0.532 0.917 1.452 2.498

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.