- Catalog number
- EK2133
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 99 101 94 94 5.34 5.7% Sample 2 294 288 315 322 302 12.49 4.1% Sample 3 963 952 841 937 923 60.01 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 99 101 94 94 5.34 5.7% Sample 1 81 99 101 94 94 5.34 5.7% Sample 2 294 288 315 322 302 12.49 4.1% Sample 2 294 288 315 322 302 12.49 4.1% Sample 3 963 952 841 937 923 60.01 6.5% Sample 3 963 952 841 937 923 60.01 6.5%
- Synonyms
- Dll1; Delta-like protein 1; Drosophila Delta homolog 1; Delta1
- Gene Name
- Dll1
- Gene ID
- 84010
- UniProt
- P97677
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q18-Q531
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 294 963 95 303 952 Mean (pg/ml) 81 294 963 95 303 952 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 Standard deviation 5.18 19.11 56.82 4.56 16.06 58.07 CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1% CV (%) 6.4% 6.5% 5.9% 4.8% 5.3% 6.1%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant rat Dll1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Delta like canonical Notch ligand 1
- Protein Name
- Delta-like protein 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05 O.D. 0.03 0.118 0.207 0.334 0.558 0.975 1.516 2.05
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
