- Catalog number
- EK2134
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene. Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 154 166 159 163 5.36 3.3% Sample 2 645 652 670 701 667 30.02 4.5% Sample 3 1669 1466 1624 1563 1581 64.8 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 171 154 166 159 163 5.36 3.3% Sample 1 171 154 166 159 163 5.36 3.3% Sample 2 645 652 670 701 667 30.02 4.5% Sample 2 645 652 670 701 667 30.02 4.5% Sample 3 1669 1466 1624 1563 1581 64.8 4.1% Sample 3 1669 1466 1624 1563 1581 64.8 4.1%
- Synonyms
- ASAH2; HNAC1; Neutral ceramidase; N-CDase; NCDase; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase [Cleaved into: Neutral ceramidase soluble form]
- Gene Name
- ASAH2
- Gene ID
- 56624
- UniProt
- Q9NR71
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: F99-I780
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 171 645 1669 147 593 1596 Mean (pg/ml) 171 645 1669 147 593 1596 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 Standard deviation 12.14 44.51 108.49 8.09 34.99 100.55 CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3% CV (%) 7.1% 6.9% 6.5% 5.5% 5.9% 6.3%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ASAH2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- N-acylsphingosine amidohydrolase 2
- Protein Name
- Neutral ceramidase
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061 O.D. 0.014 0.131 0.237 0.425 0.807 1.253 1.801 2.061
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
