- Catalog number
- EK2135
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Attractin is a protein that in humans is encoded by the ATRN gene. This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines.
- Reproducibility
- Reproducibility To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 1 1352 1388 1425 1501 1417 72.24 5.1% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 2 5864 5941 5263 5554 5656 214.91 3.8% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5% Sample 3 26013 27512 26944 25414 26471 1191.18 4.5%
- Synonyms
- ATRN; KIAA0548; MGCA; Attractin; DPPT-L; Mahogany homolog
- Gene Name
- ATRN
- Gene ID
- 8455
- UniProt
- O75882
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: A84-Q1272
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Mean (pg/ml) 1352 5864 26013 1363 6055 26542 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 Standard deviation 85.18 363.57 1586.79 94.05 387.52 1353.64 CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1% CV (%) 6.3% 6.2% 6.1% 6.9 6.4% 5.1%
- Sensitivity
- <20 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ATRN
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Attractin
- Protein Name
- Attractin
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039 O.D. 0.033 0.075 0.154 0.281 0.498 0.937 1.559 2.039
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
