- Catalog number
- EK2139
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Lysophosphatidic acid phosphatase type 6 is an acid phosphatase enzyme that is encoded in humans by the ACP6 gene. This gene encodes a member of the histidine acid phosphatase protein family. The encoded protein hydrolyzes lysophosphatidic acid, which is involved in G protein-coupled receptor signaling, lipid raft modulation, and in balancing lipid composition within the cell. Alternative splicing results in multiple transcript variants.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 28 29 24 27 0.95 3.6% Sample 2 166 157 169 174 167 4.83 2.9% Sample 3 457 479 485 460 470 14.58 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 28 29 24 27 0.95 3.6% Sample 1 25 28 29 24 27 0.95 3.6% Sample 2 166 157 169 174 167 4.83 2.9% Sample 2 166 157 169 174 167 4.83 2.9% Sample 3 457 479 485 460 470 14.58 3.1% Sample 3 457 479 485 460 470 14.58 3.1%
- Gene Name
- ACP6
- Gene ID
- 51205
- UniProt
- Q9NPH0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: CHO; Immunogen sequence: E33-E428
- Capture Antibody
- A monoclonal antibody from mouse
- Detection Antibody
- A biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human ACP6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Acid phosphatase 6, lysophosphatidic
- Protein Name
- Lysophosphatidic acid phosphatase type 6
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
