Human ACY1 ELISA Kit PicoKine®

About this product

Human ACY1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ACY1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2140

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aminoacylase-1 is an enzyme that in humans is encoded by the ACY1 gene. This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 457 488 462 454 465 15.35 3.3% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 3 8622 8845 8961 8753 8795 448.56 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 457 488 462 454 465 15.35 3.3% Sample 1 457 488 462 454 465 15.35 3.3% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 3 8622 8845 8961 8753 8795 448.56 5.1% Sample 3 8622 8845 8961 8753 8795 448.56 5.1%

Gene Name

ACY1

Gene ID

95

UniProt

Q03154

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-S408

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6%

Sensitivity

<20 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ACY1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.