- Catalog number
- EK2144
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Bst1 (Bone marrow stromal cell antigen 1, ADP-ribosyl cyclase 2, CD157) is an enzyme that in humans is encoded by the BST1 gene. Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 3 5140 5265 5333 5441 5295 232.97 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 3 5140 5265 5333 5441 5295 232.97 4.4% Sample 3 5140 5265 5333 5441 5295 232.97 4.4%
- Gene Name
- BST1
- Gene ID
- 683
- UniProt
- Q10588
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G29-A293
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Biotinylated polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA)
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Specificity
- Natural and recombinant human BST1
- Anticoagulant
- Heparin or EDTA
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
