Human CD109 ELISA Kit PicoKine®

About this product

Human CD109 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD109 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2147

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a glycosyl phosphatidylinositol (GPI) -linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta) . Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 470 489 505 483 21.74 4.5% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 3 4527 4652 4741 4426 4587 288.95 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 470 489 505 483 21.74 4.5% Sample 1 468 470 489 505 483 21.74 4.5% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 3 4527 4652 4741 4426 4587 288.95 6.3% Sample 3 4527 4652 4741 4426 4587 288.95 6.3%

Gene Name

CD109

Gene ID

135228

UniProt

Q6YHK3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V22-S1268

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6%

Sensitivity

<20 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD109

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.