Human CTGF ELISA Kit PicoKine®

About this product

Human CTGF ELISA Kit PicoKine® (96 Tests) . Quantitate Human CTGF in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2149

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 61 48 55 51 1.85 3.6% Sample 2 153 167 185 172 169 6.77 4% Sample 3 695 701 755 786 734 38.18 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 61 48 55 51 1.85 3.6% Sample 1 41 61 48 55 51 1.85 3.6% Sample 2 153 167 185 172 169 6.77 4% Sample 2 153 167 185 172 169 6.77 4% Sample 3 695 701 755 786 734 38.18 5.2% Sample 3 695 701 755 786 734 38.18 5.2%

Gene Name

CCN2

Gene ID

1490

UniProt

P29279

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q27-A349

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 153 695 45 166 726 Standard deviation 2.3 8.72 34.06 2.75 9.13 28.31 CV (%) 5.6% 5.7% 4.9% 6.1% 5.5% 3.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 153 695 45 166 726 Standard deviation 2.3 8.72 34.06 2.75 9.13 28.31 CV (%) 5.6% 5.7% 4.9% 6.1% 5.5% 3.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 41 153 695 45 166 726 Mean (pg/ml) 41 153 695 45 166 726 Standard deviation 2.3 8.72 34.06 2.75 9.13 28.31 Standard deviation 2.3 8.72 34.06 2.75 9.13 28.31 CV (%) 5.6% 5.7% 4.9% 6.1% 5.5% 3.9% CV (%) 5.6% 5.7% 4.9% 6.1% 5.5% 3.9%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CCN2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.106 0.141 0.213 0.372 0.61 1.128 1.915 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.106 0.141 0.213 0.372 0.61 1.128 1.915 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.106 0.141 0.213 0.372 0.61 1.128 1.915 O.D. 0.056 0.106 0.141 0.213 0.372 0.61 1.128 1.915

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.