Human CD7 ELISA Kit PicoKine®

About this product

Human CD7 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD7 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK2150

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 23 25 28 25 0.81 3.3% Sample 2 165 157 164 163 162 7.79 4.8% Sample 3 451 458 462 473 461 20.28 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 23 25 28 25 0.81 3.3% Sample 1 22 23 25 28 25 0.81 3.3% Sample 2 165 157 164 163 162 7.79 4.8% Sample 2 165 157 164 163 162 7.79 4.8% Sample 3 451 458 462 473 461 20.28 4.4% Sample 3 451 458 462 473 461 20.28 4.4%

Gene Name

CD7

Gene ID

924

UniProt

P09564

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: HEK293; Immunogen sequence: A26-P180

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 165 451 26 177 426 Standard deviation 1.21 11.39 32.02 1.4 11.15 28.12 CV (%) 5.5% 6.9% 7.1% 5.1% 6.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 165 451 26 177 426 Standard deviation 1.21 11.39 32.02 1.4 11.15 28.12 CV (%) 5.5% 6.9% 7.1% 5.1% 6.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 165 451 26 177 426 Mean (pg/ml) 22 165 451 26 177 426 Standard deviation 1.21 11.39 32.02 1.4 11.15 28.12 Standard deviation 1.21 11.39 32.02 1.4 11.15 28.12 CV (%) 5.5% 6.9% 7.1% 5.1% 6.3% 6.6% CV (%) 5.5% 6.9% 7.1% 5.1% 6.3% 6.6%

Sensitivity

<10 pg/mL

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD7

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.113 0.159 0.212 0.33 0.571 1.027 1.832 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.113 0.159 0.212 0.33 0.571 1.027 1.832 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.113 0.159 0.212 0.33 0.571 1.027 1.832 O.D. 0.061 0.113 0.159 0.212 0.33 0.571 1.027 1.832

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.